Journal: eBioMedicine

Article Title: Preclinical characterisation of the protective capacity of an anti-nucleoprotein hRSV monoclonal antibody

doi: 10.1016/j.ebiom.2025.106104

Figure Lengend Snippet: The humanised anti-N-hRSV mAbs demonstrate antibody-dependent cellular cytotoxicity and complement-dependent cytotoxicity activity in vitro . A. The percentage of antibody-dependent cellular cytotoxicity (ADCC) activity for each clone of the humanised anti-N-hRSV mAbs (clones P1-04H, P1-05D, P2-01A, and P2-01D) was measured using luciferase expression levels in Jurkat NFAT-luc FcγRIII cells incubated with the humanised anti-N-hRSV mAbs against N-hRSV. B. The percentage of complement-dependent cytotoxicity (CDC) activity for each clone was assessed by viability assay of HEp-2 cells infected with hRSV-GFP, treated with the four clones of the humanised anti-N-hRSV mAbs, followed by the addition of mouse purified complement. Three independent experiments were conducted for each ADCC and CDC assay (N = 3).

Article Snippet: After 30 min of antibody incubation, 100 μl of a 1:25 solution of mouse-purified complement (Rockland Immunochemical; cat #R.C201-0005) was added, bringing the final concentration in the well to 1:50, and the plate was incubated for 3 h at 37 °C with 5% CO 2 .

Techniques: Activity Assay, In Vitro, Clone Assay, Luciferase, Expressing, Incubation, Viability Assay, Infection, Purification, CDC Assay

Journal: Journal of the American Chemical Society

Article Title: Biosynthesis of 14-membered cyclopeptide alkaloids via non-heme-iron- and 2-oxoglutarate-dependent oxidative decarboxylation

doi: 10.1021/jacs.5c23400

Figure Lengend Snippet: ( A ) EIC traces of 3 and decarboxylated cyFPIY ( 4 ) in methanolic extracts of transgenic N. benthamiana leaves expressing ZjuPrec-(FPIY) 3 -ZjuBURP with or without candidate decarboxylase ZjuDC for 7 days. ( B ) In vitro reconstitution of ZjuDC activity in the presence of Fe(II) and 2OG. ( C ) In vitro cofactor and substrate screening assays of ZjuDC. ( D ) ZjuDC reaction. ( E ) Kinetic characterization of ZjuDC with substrate 3 . Abbreviations: 2OG - 2-oxoglutarate, EIC – extracted ion chromatogram. ( F ) ZjuDC reaction with dimethylated substrate N,N -Me 2 -cyFPIY ( 6 ).

Article Snippet: Assays were 50 μL total volume with final concentrations of 50 mM HEPES pH 7.5, 200 μM FeSO 4 -7H 2 O, 500 μM 2-oxoglutarate (Chem-Impex Int’l Inc.), 400 μM L-ascorbic acid sodium salt (Acros Organics), 4% dimethyl sulfoxide, and 90 μM substrate 3 .

Techniques: Transgenic Assay, Expressing, In Vitro, Activity Assay

Journal:

Article Title: Flow Cytometry Compared with Indirect Immunofluorescence for Rapid Detection of Dengue Virus Type 1 after Amplification in Tissue Culture

doi: 10.1128/JCM.39.10.3672-3677.2001

Figure Lengend Snippet: Detection of DEN-1 in C6/36 cells infected at different MOIs on day 1 postinoculation using indirect IF stain

Article Snippet: After confluent growth of C6/36 cells in 96-well plates (Costar), DEN-1 (Hawaii strain) and two local DEN-1 isolates (766733 and 066267) were inoculated and incubated at 28°C.

Techniques: Infection

Journal:

Article Title: Flow Cytometry Compared with Indirect Immunofluorescence for Rapid Detection of Dengue Virus Type 1 after Amplification in Tissue Culture

doi: 10.1128/JCM.39.10.3672-3677.2001

Figure Lengend Snippet: Comparison of three fixation-permeabilization methods for the detection of dengue virus in C6/36 cells using flow cytometry a

Article Snippet: After confluent growth of C6/36 cells in 96-well plates (Costar), DEN-1 (Hawaii strain) and two local DEN-1 isolates (766733 and 066267) were inoculated and incubated at 28°C.

Techniques: Flow Cytometry

Journal:

Article Title: Flow Cytometry Compared with Indirect Immunofluorescence for Rapid Detection of Dengue Virus Type 1 after Amplification in Tissue Culture

doi: 10.1128/JCM.39.10.3672-3677.2001

Figure Lengend Snippet: Comparison of three MAbs used for the detection of DEN-1 virus (Hawaii) antigen in C6/36 cells using flow cytometry a

Article Snippet: After confluent growth of C6/36 cells in 96-well plates (Costar), DEN-1 (Hawaii strain) and two local DEN-1 isolates (766733 and 066267) were inoculated and incubated at 28°C.

Techniques: Flow Cytometry

Journal:

Article Title: Flow Cytometry Compared with Indirect Immunofluorescence for Rapid Detection of Dengue Virus Type 1 after Amplification in Tissue Culture

doi: 10.1128/JCM.39.10.3672-3677.2001

Figure Lengend Snippet: Flow cytometric histograms of DEN-1 (Hawaii strain)-infected C6/36 cells stained with DEN-1 MAb 16-4 (anti-NS1) (black) overlaid with histograms of mock-infected cells (white). C6/36 mosquito cells were infected with DEN-1 (Hawaii) at an MOI of 0.01. (A through G) Different time points postinfection. FL1-H, FITC fluorescence intensity. The bars represent the proportion of positive cells. (H) Detection of DEN-1 viral RNA in infected C6/36 cells collected at different time points postinfection using RT-nPCR. Lanes 1 to 8 show results at 8, 12, 16, 24, 26, 30, 48, and 72 h postinfection, respectively. Lane 9, mock-infected C6/36 cells; lane 10, culture medium only; lane 11, DEN-1 (Hawaii) virus; lane 12, reagent control; lane M, molecular size markers.

Article Snippet: After confluent growth of C6/36 cells in 96-well plates (Costar), DEN-1 (Hawaii strain) and two local DEN-1 isolates (766733 and 066267) were inoculated and incubated at 28°C.

Techniques: Infection, Staining, Fluorescence

Journal:

Article Title: Flow Cytometry Compared with Indirect Immunofluorescence for Rapid Detection of Dengue Virus Type 1 after Amplification in Tissue Culture

doi: 10.1128/JCM.39.10.3672-3677.2001

Figure Lengend Snippet: Comparison of the sensitivity in detection of DEN-1 virus (Hawaii)-infected C6/36 cells by conventional IF staining and flow cytometry a

Article Snippet: After confluent growth of C6/36 cells in 96-well plates (Costar), DEN-1 (Hawaii strain) and two local DEN-1 isolates (766733 and 066267) were inoculated and incubated at 28°C.

Techniques: Staining, Flow Cytometry